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Trans-Pacific-RAD-Seq Population Genomics Confirms Introgressive Hybridization in Eastern Pacific Pocillopora Corals
Discrepancies between morphology-based taxonomy and phylogenetic systematics are common in Scleractinian corals. In Pocillopora corals, nine recently identified genetic lineages disagree fundamentally with the 17 recognized Pocillopora species, including 5 major Indo-Pacific reef-builders. Pocillopora corals hybridize in the Tropical Eastern Pacific, so it is possible that some of the disagreement between the genetics and taxonomy […]
We describe a deep-sequencing procedure for tracking large numbers of transposon mutants of Pseudomonas aeruginosa. The procedure employs a new Tn-seq methodology based on the generation and amplification of single-strand circles carrying transposon junction sequences (the Tn-seq circle method), a method which can be used with virtually any transposon. The procedure reliably identified more than […]
Single cell genomics is a powerful and increasingly popular tool for studying the genetic make-up of uncultured microbes. A key challenge for successful single cell sequencing and analysis is the removal of exogenous DNA from whole genome amplification reagents. We found that UV irradiation of the multiple displacement amplification (MDA) reagents, including the Phi29 polymerase […]
Paternally-induced transgenerational environmental reprogramming of metabolic gene expression in mammals
Epigenetic information can be inherited through the mammalian germline, and represents a plausible transgenerational carrier of environmental information. To test whether transgenerational inheritance of environmental information occurs in mammals, we carried out an expression profiling screen for genes in mice that responded to paternal diet.
2012 HUPO Poster- The Yin-Yang of Sample Preparation: Reagent optimization for maximal yield, activity, and preservation of biomarkers using Adaptive Focused Acoustics (AFA)
The Yin-Yang of Sample Preparation: Reagent optimization for maximal yield, activity, and preservation of biomarkers using Adaptive Focused Acoustics (AFA)
Transitioning from custom amplicon-based parallel sequencing to hybrid capture-based sequencing in molecular pathology routine diagnostics
(Application Notes )
Eloe-Fadrosh et. al. mBio 2015 April v6 no2
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Covaris provides tools and technologies to improve pre-analytical sample preparation, enable novel drug formulations, and manage compounds in the drug discovery process.