Genetic analysis from DBS cards has become routine in many laboratories, but the potential offered by NGS to deliver a wealth of addition clinically useful information is not yet fully recognized. As sequencing costs come down in clinical laboratories, the barrier to obtaining sufficient DNA of high quality for library preparation remains.
Covaris has developed the truXTRAC™ DBS kit to deliver high-quality, unbiased sequencing results from a single 3 mm DBS punch. The protocol is fully optimized for the clinical implementation and is compatible with automation for high-throughput processing.
truXTRAC DBS delivers substantially higher yields and concentrations of DNA than other methods making it directly compatible with NGS library prep.
truXTRAC DBS extracted DNA has superior performance in PCR amplification making it perfect for NGS library construction. Covaris truXTRAC samples amplified well in qPCR with significantly higher yields than samples processed using QIAGEN preparation.
DBS DNA samples were purified and then their concentration was determined by Qubit fluorimetry
Normalized 0.5 ng and 2.0 ng template aliquots were input to qPCR (67 bp. amplicon) and quantified with respect to a hgDNA standard curve. QIAGEN samples were pooled for qPCR due to lower yield, so no error bars are shown.
the truXTRAC DBS workflow integrates Covaris AFA DNA shearing to your chosen fragment size. For applications requiring even more DNA we offer multi-punch options with microTUBE 500. Proteomics applications are also in development.