truChIP™ Chromatin Shearing Kits

for Chromatin Immunoprecipitation

truChIP™ Chromatin Shearing Kits provide optimized, flexible, and universal protocols for chromatin shearing with high performance Focused-ultrasonicators, enabling the seamless inclusion of truChIP chromatin shearing into any Chromatin Immunoprecipitation (ChIP) protocol. truChIP kits bring the advantages of Focused-ultrasonication directly to ChIP assays by increasing sensitivity, yield, and reproducibility.

truChIP chromatin shearing kits are available in multiple configurations optimized for specific ChIP assays, including:

truChIP Chromatin shearing kits:



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[tab title=”Products”]
truChIP™Kits AFA Optimized

truChIP Ultra-low cell chromatin shearing reagent kit (PN520156)
The kit contains reagents required for high-yield and high-quality chromatin shearing.

  • Optimized for processing ≤100K cells

truChIP Chromatin shearing reagent kit (PN 520154)
The kit contains reagents required for high-yield and high-quality chromatin shearing.

  • Includes low-cell protocol optimized for processing 0.5-3 million cells in 130 µl shearing volume
  • Includes high-cell protocol optimized for processing 5-30 million cells in 1 ml shearing volume

truChIP Tissue Chromatin Shearing Kit SDS (PN 520083)
The kit contains reagents required for high-yield and high-quality chromatin shearing.

  • Includes low tissue mass optimized protocol for processing up to 20 mg of tissue in 130 µl shearing volume
  • Includes high tissue mass optimized protocol for processing up to 120 mg of tissue in 1 ml shearing volume
truChIP Kits AFA Focused-ultrasonicators
M220 S220 E220evolution E220 LE220
truChIP Ultra-low cell chromatin shearing reagent kit
(PN 520156)
M220 Holder XTU
(PN 500414)

M220 Holder XTU insert microTUBE-130 µl
(PN 500489)

Holder microTUBE
(PN 500114)
Rack 12 Place 8 microTUBE Strip V2
(PN 500444)
Rack 96 place microTUBE
(PN 500282)

Rack 12 place 8 microTUBE strip
(PN 500191)

96 microTUBE Plate
(PN 520078)

Rack 96 place microTUBE
(PN 500282)

Rack 12 place 8 microTUBE strip
(PN 500191)

96 microTUBE Plate
(PN 520078)

truChIP Chromatin shearing reagent kit
(PN 520154)
Low Cell Protocol
M220 Holder XTU
(PN 500414)

M220 Holder XTU insert microTUBE-130 µl
(PN 500489)

Holder microTUBE
(PN 500114)
Rack 12 Place 8 microTUBE Strip V2
(PN 500444)
Rack 96 place microTUBE
(PN 500282)

Rack 12 place 8 microTUBE strip
(PN 500191)

96 microTUBE Plate
(PN 520078)

Rack 96 place microTUBE
(PN 500282)

Rack 12 place 8 microTUBE strip
(PN 500191)

Rack 24 place milliTUBE 1ml
(PN 500368)

truChIP Chromatin shearing reagent kit
(PN 520154)
High-Cell Protocol
M220 Holder XTU
(PN 500414)

M220 Holder XTU insert milliTUBE 1ml
(PN 500422)

Holder milliTUBE 1ml
(PN 500371)
Rack E220e 4 place milliTUBE 1 ml
(PN 500431)
Rack 24 Place milliTUBE 1 ml
(PN 500368)
Rack 24 Place milliTUBE 1 ml
(PN 500368)
truChIP Tissue Chromatin Shearing Kit SDS
(PN 520083)
Low Tissue Amount

* Requires CP02

M220 Holder XTU
(PN 500414)

M220 XTU Insert microTUBE-130 µl
(PN 500489)

Holder microTUBE
(PN 500114)
Rack 12 Place 8 microTUBE Strip V2
(PN 500444)
Rack 96 place microTUBE
(PN 500282)

Rack 12 place 8 microTUBE strip
(PN 500191)

96 microTUBE plate
(PN 520078)

Rack 96 place microTUBE
(PN 500282)

Rack 12 place 8 microTUBE strip
(PN 500191)

96 microTUBE plate
(PN 520078)

truChIP Tissue Chromatin Shearing Kit SDS
(PN 520083)
High Tissue Amount

* Requires CP02

M220 Holder XTU
(PN 500414)

M220 Holder XTU Holder Insert for the milliTUBE 1 ml
(PN 500422)

Holder milliTUBE 1ml
(PN 500371)
Rack E220e 4 place milliTUBE 1 ml
(PN 500431)
Rack 24 Place milliTUBE 1 ml
(PN 500368)
Rack 24 Place milliTUBE 1 ml
(PN 500368)
Complementary Products:

cryoPREP™ Extraction Systems
* cryoPREP Impactors
tissueTUBE Devices

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[tab title=”Protocols”]

truChIP™ Protocols

truChIP™ ultra low-cell chromatin shearing reagent kit
truChIP™ chromatin shearing reagents kit
truChIP™ tissue chromatin shearing kit

cryoPREP

Tissue Dry Pulverization

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[tab title=”References”]

Application Notes

truChip Fixation Optimization App Note
Broad Yeast Application Note
truChIP poster

Selected References

Repression of Androgen Receptor Transcription through the E2F1/DNMT1 Axis. Conrad David Valdez, Joanne N. Davis, Hana M. Odeh, Tristan L. Layfield, Craig S. Cousineau, Thomas R. Berton, David G. Johnson, Kirk J. Wojno, Mark L. Day. PLoS One. 2011; 6(9): e25187. Published online 2011 September 26. doi: 10.1371/journal.pone.0025187. PMCID: PMC3180375
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180375/?tool=pmcentrez

Phenobarbital Mediates an Epigenetic Switch at the Constitutive Androstane Receptor (CAR) Target Gene Cyp2b10 in the Liver of B6C3F1 Mice. Harri Lempiäinen, Arne üller, Sarah Brasa, Soon-Siong Teo, Tim-Christoph Roloff, Laurent Morawiec, Natasa Zamurovic, Axel Vicart, Enrico Funhoff, Philippe Couttet, Dirk Schübeler, Olivier Grenet, Jennifer Marlowe, Jonathan Moggs, Rémi Terranova. PLoS One. 2011; 6(3): e18216. Published online 2011 March 24. doi: 10.1371/journal.pone.0018216. PMCID: PMC3063791
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3063791/?tool=pmcentrez

Efficient Double Fragmentation ChIP-seq Provides Nucleotide Resolution Protein-DNA Binding Profiles. Michal Mokry, Pantelis Hatzis, Ewart de Bruijn, Jan Koster, Rogier Versteeg, Jurian Schuijers, Marc van de Wetering, Victor Guryev, Hans Clevers, Edwin Cuppen. PLoS One. 2010; 5(11): e15092. Published online 2010 November 30. doi: 10.1371/journal.pone.0015092. PMCID: PMC2994895
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2996544/?tool=pmcentrez

Stable S/MAR-based episomal vectors are regulated at the chromatin level. Federico Tessadori, Kang Zeng, Erik Manders, Martijn Riool, Dean Jackson, Roel van Driel. Chromosome Res. 2010 November; 18(7): 757-775. Published online 2010 November 16. doi: 10.1007/s10577-010-9165-4. PMCID: PMC2996544
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2996544/?tool=pmcentrez

ZBED6, a Novel Transcription Factor Derived from a Domesticated DNA Transposon Regulates IGF2 Expression and Muscle Growth. Ellen Markljung, Lin Jiang, Jacob D. Jaffe, Tarjei S. Mikkelsen, Ola Wallerman, Martin Larhammar, Xiaolan Zhang, Li Wang, Veronica Saenz-Vash, Andreas Gnirke, Anders M. Lindroth, Romain Barrés, Jie Yan, Sara Strömberg, Sachinandan De, Fredrik Pontén, Eric S. Lander, Steven A. Carr, Juleen R. Zierath, Klas Kullander, Claes Wadelius, Kerstin Lindblad-Toh, Göran Andersson, Göran Hjälm, Leif Andersson. PLoS Biol. 2009 December; 7(12): e1000256. Published online 2009 December 15. doi: 10.1371/journal.pbio.1000256. PMCID: PMC2780926
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2780926/?tool=pmcentrez

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