M220 Focused-ultrasonicator

Protocols for Ion Torrent Platforms

Covaris M220 has been tested and validated by Ion Torrent for use with their sequencing platforms. Covaris has developed DNA shearing protocols compatible with the Ion Xpress Plus gDNA Fragment Library Preparation Kit.

Download protocol (pdf)

100 bp read – Bioanalyzer analysis of DNA fragmented with the Covaris M220 Focused-ultrasonicator
1 µg of lambda DNA in 50 µl low TE was fragmented with the Covaris M220 in a Screw-Cap microTUBE for 375 seconds. Analysis was with the Agilent High Sensitivity DNA Kit. Peaks at 35 and 10380 bp represent low- and high-molecular weight markers.
200 bp read – Bioanalyzer analysis of DNA fragmented with the Covaris M220 Focused-ultrasonicator
1 µg of lambda DNA in 50 µl low TE was fragmented with the Covaris M220 in a Screw-Cap microTUBE for 130 seconds. Analysis was with the Agilent High Sensitivity DNA Kit. Peaks at 35 and 10380 bp represent low- and high-molecular weight markers.
300 bp read – Bioanalyzer analysis of DNA fragmented with the Covaris M220 Focused-ultrasonicator
1 µg of lambda DNA in 50 µl low TE was fragmented with the Covaris M220 in a Screw-Cap microTUBE for 100 seconds. Analysis was with the Agilent High Sensitivity DNA Kit. Peaks at 35 and 10380 bp represent low- and high-molecular weight markers.
400 bp read – Bioanalyzer analysis of DNA fragmented with the Covaris M220 Focused-ultrasonicator
1 µg of lambda DNA in 50 µl low TE was fragmented with the Covaris M220 in a Screw-Cap microTUBE for 60 seconds. Analysis was with the Agilent High Sensitivity DNA Kit. Peaks at 35 and 10380 bp represent low- and high-molecular weight markers.

 

Note: The parameters differ from the settings published in our Quick Guide: DNA shearing with M220 Focused-ultrasonicator. Our Quick Guide has been developed using the Agilent 12k DNA Kit and the fragment size distribution representations are not identical between this kit and the Agilent High Sensitivity DNA Kit. We recommend, Ion Torrent users follow the settings developed specifically for Ion Torrent platforms.