DNA Shearing for Next-Generation Sequencing
• Proven Covaris AFA shearing process, as used in genome centers worldwide
• Generates tight, highly reproducible fragment distributions
• Optimized shearing programs to generate fragment sizes between 150 and 5,000 bp
• Also effective for low-mass, high epitope recovery chromatin shearing
• Now the “Scientist’s Standard” is in a low-cost, compact, easy to use design
M220 Focused-ultrasonicator™ is designed for Next-Gen Sequencing applications requiring fragment sizes between 150bp and 5kb. Compact size and ease-of-use make the M220 Focused-ultrasonicator the ideal DNA shearing solution for MiSeq™ and PGM™ users.
The M220 runs a variety of DNA shearing protocols with Covaris SonoLab 7 software and uses both the Covaris microTUBE™ & miniTUBE™.
Integrated temperature control within the M220 eliminates the need for an external chiller, saving space and cost.
Covaris AFA (Adaptive Focused Acoustics™) transmits acoustic energy at ultrasonic frequencies (>400KHz) and very short wavelengths (~3mm) and is uniquely able to focus the energy into your sample. AFA controls the shearing forces that fragment nucleic acids to the selected size. The process is isothermal, non contact, and quiet.
Covaris AFA technology in the M220 eliminates operator induced variations, improves recoveries, increases efficiency, and provides standardized results.
|Controlled energy delivery||User selectable fragment lengths|
|Isothermal processing||No heat damage, high sample recovery|
|Non-contact, closed vessel||No cross contamination, no aerosols, no clean up|
|Quiet operation||No discomfort to operators|
|Robust and reliable design||Reproducible results, year after year|
|Tried and tested in major genome centers worldwide||The mechanical shearing method of choice|
The M220 Focused-ultrasonicator provides the same high-performance shearing results researchers have come to expect from Covaris S-Series & E-Series instruments. As demonstrated below, the M220 Focused-ultrasonicator reproducibly shears DNA from 150bp to 5kb in microTUBEs and miniTUBEs. See also our Application Note: DNA Shearing for NGS with the M220 Focused-ultrasonicator.
Tubes and vessels specifically engineered for AFA
Holders and Inserts for M220 Focused-ultrasonicator
M220 Holder XTU (PN 500414). This holder requires an insert specific to the AFA vessels used.
M220 Holder XTU Insert microTUBE 130 µl (PN 500489). For use with microTUBE AFA Fiber Snap-Cap, truXTRAC FFPE Kits and truChIP Kits.
M220 Holder XTU Insert microTUBE 50 µl (PN 500488). For use with microTUBE-50 AFA Fiber Screw-Cap.
M220 Holder XTU Insert microTUBE 15 µl (PN 500420). For use with microTUBE-15 AFA Beads Screw-Cap.
M220 Holder XTU Insert milliTUBE 1 ml (PN 500422). For use with milliTUBE 1 ml and truChIP Kits.
M220 Holder miniTUBE (PN 500302). This holder is compatible with miniTUBE Clear, Blue and Red
Cleaning and Maintenance
M220 Fill and Drain Accessory Kit
Quick Guide: DNA Shearing with M220 Focused-ultrasonicator and Holder XTU (*)
(*) This protocol has been updated to include microTUBE-50, optimized for 50 µl samples. For superseded version of DNA Shearing protocols, please see below:
Quick Guide: DNA shearing with M220 Focused-ultrasonicator (rev E, April 2013)
|Regulatory Certifications||CE, ETL Mark (for Product Safety), WEEE|
|Size||33 (width) 38.7 (depth) 27.3 (height) centimeters|
|Operating environment||Laboratory, 15ºC – 25ºC ambient|
|Sample vessels||microTUBE, microTUBE-50, milliTUBE 1 ml and miniTUBE|
|Maximum power||75W PIP (Peak Incident Power)|
|Temperature control||Internal temperature control: Protocol temperature range from 7ºC to 20ºC|
|Applications||DNA shearing 150 bp to 5 kb|
|System Control||Notebook computer interface via USB, operating Microsoft Windows XP SP3 or 7 Professional, with Covaris SonoLab 7 control software installed.|
|Installation / setup||Customer installable|
|Safety||Complies with Low Voltage Directive 2006/95/EC. Certified to IEC/EN/UL 61010-1:2004 “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part1: General Requirements”|
|EMC||Complies with radio frequency emissions and immunity requirements for Class A Industrial/Scientific/Medical (ISM) equipment under EN 61326-1:2005, as well as EN 61000-3-2:2004 and EN61000-3-3:1995. These standards meet the essential requirements of the EU EMC Directive 2004/108/EC. Additionally, the instrument meets FCC Part 15 Class A radio emissions requirements for the USA and ICES-003 Class A for Industry Canada.|
Ion Torrent and PGM™ are registered trademarks of Ion Torrent Systems, Inc.
Illumina® and MiSeq are registered trademarks of Illumina, Inc.