M220 Focused-ultrasonicator™

DNA Shearing for Next-Generation Sequencing

• Proven Covaris AFA shearing process, as used in genome centers worldwide
• Generates tight, highly reproducible fragment distributions
• Optimized shearing programs to generate fragment sizes between 150 and 5,000 bp
• Also effective for low-mass, high epitope recovery chromatin shearing
• Now the “Scientist’s Standard” is in a low-cost, compact, easy to use design

evaluation

M220 Focused-ultrasonicator™ is designed for Next-Gen Sequencing applications requiring fragment sizes between 150bp and 5kb. Compact size and ease-of-use make the M220 Focused-ultrasonicator the ideal DNA shearing solution for MiSeq™ and PGM™ users.

The M220 runs a variety of DNA shearing protocols with Covaris SonoLab 7 software and uses both the Covaris microTUBE™ & miniTUBE™.

Integrated temperature control within the M220 eliminates the need for an external chiller, saving space and cost.

Covaris AFA (Adaptive Focused Acoustics™) transmits acoustic energy at ultrasonic frequencies (>400KHz) and very short wavelengths (~3mm) and is uniquely able to focus the energy into your sample. AFA controls the shearing forces that fragment nucleic acids to the selected size. The process is isothermal, non contact, and quiet.

Covaris AFA technology in the M220 eliminates operator induced variations, improves recoveries, increases efficiency, and provides standardized results.

Acoustics by Covaris™ provides
Controlled energy delivery User selectable fragment lengths
Isothermal processing No heat damage, high sample recovery
Non-contact, closed vessel No cross contamination, no aerosols, no clean up
Quiet operation No discomfort to operators
Robust and reliable design Reproducible results, year after year
Tried and tested in major genome centers worldwide The mechanical shearing method of choice
New “Single Box” Design
The M220 is designed with a small footprint for bench-top use. The focused-acoustic block has been optimized to reduce its size, and the Peltier-effect thermoelectric temperature controller is fully integrated. The notebook computer operating the SonoLab software is located on top of the instrument. As M220 operates in the ultrasonic range, very little sound is audible to the operator and so it can be comfortably used anywhere in the lab.
Focused-ultrasonicator Assembly
Each focused-ultrasonicor is calibrated by Covaris to ensure uniform performance across all instruments and allowing standardized protocols. Only 15ml of AFA-grade Water is needed to couple acoustic energy to the sample. An integrated Water Level Sensor detects when water needs to be added.
Highly Accurate Temperature Control
The SonoLab software utilizes an optimized algorithm to drive the Peltier-effect thermoelectric controller which provides precise control of the temperature regardless of the acoustic treatment. The temperature stays within +/- 1°C of the set-point during a typical 10 W average power treatment allowing true isothermal processing of your samples.
Real-Time Process Monitoring
SonoLab software gives a real time display of the parameters relevant to your acoustic treatment such as Bath Temperature, Average Power, Instrument Status, and Elapsed/ Remaining Time.
Preloaded Protocols
SonoLab comes with preloaded protocols for common applications. It includes DNA shearing protocols for 50 and 130 μl sample and for fragment size distribution ranging from 100 to 5,000 bp as well as Chromatin shearing protocols. Updated protocols can be downloaded from the Covaris Website.
Integrated Quality Control
SonoLab comes with a History Tab. For every sample processed, Date and Time, User Name, AFA Settings, actual Power delivered, Bath Temperature, and customizable Sample Notes are recorded so sample tracking is easy.

 

The M220 Focused-ultrasonicator provides the same high-performance shearing results researchers have come to expect from Covaris S-Series & E-Series instruments. As demonstrated below, the M220 Focused-ultrasonicator reproducibly shears DNA from 150bp to 5kb in microTUBEs and miniTUBEs. See also our Application Note: DNA Shearing for NGS with the M220 Focused-ultrasonicator.

Versatile DNA Shearing Performance
Figure 1 demonstrates M220 DNA shearing performance for fragment lengths from 150bp to 1.5kb in the microTUBE, and to 2, 3 and 5 kbp in the miniTUBE. The M220 provides the same versatile DNA shearing capabilities as the S220, E220, & LE220.
High reproducibility at 300bp DNA Shearing
Figure 2 displays the reproducibility of the M220 when shearing multiple DNA fragments to a fragment length of 300bp by the same M220. The M220 shearing performance is highly reproducible. DNA was sheared using standard Covaris microTUBEs.
Consistent 3kb Fragment Length DNA Shearing
Figure 3 demonstrates M220 shearing performance a fragment length of 3kb. Multiple samples were reproducibly sheared to 3kb using Covaris Blue miniTUBEs. The M220 provides consistent DNA shearing performance day-to-day and lab-to-lab.

Tubes and vessels specifically engineered for AFA


Screw-Cap microTUBE for 50µl volume – from 150bp to 800bp fragments
(Order online)


Snap-Cap microTUBE for 130µl volume – from 150bp to 1,500bp fragments
(Order online)


miniTUBE Clear, Blue and Red for 2, 3 and 5 kbp fragments
(Order online)

Accessories for M220 Focused-ultrasonicator
M220 Holder microTUBE. This holder is compatible with microTUBE Snap-Cap and Screw-Cap
(Order online)
M220 Holder miniTUBE. This holder is compatible with miniTUBE Clear, Blue and Red
(Order online)
M220 Holder-XT microTUBE. High performance holder for ChIP and FFPE applications. Compatible with microTUBE Snap-Cap
(Order online)
miniTUBE Prep Station and Stand. Allows the sample to be easily loaded. A tilted stand help recover the entire sample
(Order online)
microTUBE Prep Station and Tool. Allows the sample to be easily loaded in microTUBE Snap and Screw-Cap.
(Order online)
M220 Accessories Storage Station.Storage station for 3 M220 Holders with place for AFA-grade water bottle and cleaning syringe.
(Order online)
Cleaning and Maintenance
AFA-Grade Water
(Order online)

M220 Fill and Drain Accessory Kit
(Order online)
M220 Swab Cleaning Kit
(Order online)

Product Specifications
Regulatory Certifications CE, ETL Mark (for Product Safety), WEEE
Size 33 (width) 38.7 (depth) 27.3 (height) centimeters
Weight 15 kg
Operating environment Laboratory, 15ºC – 25ºC ambient
Sample vessels Snap-Cap microTUBE, Screw-Cap microTUBE and Clear miniTUBE, Blue miniTUBE, Red miniTUBE
Maximum power 75W PIP (Peak Incident Power)
Temperature control Internal temperature control: Protocol temperature range from 7ºC to 20ºC
Applications DNA shearing 150 bp to 5 kb
Software SonoLab 7
System Control Notebook computer interface via USB, operating Microsoft Windows XP SP3 or 7 Professional, with Covaris SonoLab 7 control software installed.
Installation / setup Customer installable
Safety Complies with Low Voltage Directive 2006/95/EC. Certified to IEC/EN/UL 61010-1:2004 “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part1: General Requirements”
EMC Complies with radio frequency emissions and immunity requirements for Class A Industrial/Scientific/Medical (ISM) equipment under EN 61326-1:2005, as well as EN 61000-3-2:2004 and EN61000-3-3:1995. These standards meet the essential requirements of the EU EMC Directive 2004/108/EC. Additionally, the instrument meets FCC Part 15 Class A radio emissions requirements for the USA and ICES-003 Class A for Industry Canada.


Ion Torrent and PGM™ are registered trademarks of Ion Torrent Systems, Inc.
Illumina® and MiSeq are registered trademarks of Illumina, Inc.