The LE220 Focused-ultrasonicator is a versatile system engineered for high throughput pre-analytical sample processing by Adaptive Focused Acoustics™. The LE220 utilizes a unique line-shaped transducer and computer controlled electronics to deliver highly controlled acoustic energy to the focal zone. The LE220 Focused-ultrasonicator delivers the same reproducibility as the renowned S-Series and E-Series AFA Instruments, so you can be assured that tube-to-tube and row-to-row precision is excellent.
The LE220 is specifically designed for operations requiring a higher throughput Covaris AFA solution. With highly reproducible performance characteristics matching Covaris S-Series and E-Series instruments, the LE220 applies controlled acoustic energy to multiple samples at one time. For example, in a 96 microTUBE Plate, a row of 8 samples is treated simultaneously by the LE220’s line transducer. By indexing all 12 rows across the acoustic field, 96 samples are processed 8X faster than with a single-sample instrument. The LE220 capably manages a wide variety of sample formats in an SBS microplate footprint (including 8, 24, 48, 96), processing an entire row at a time.
Key applications for the LE220 include: DNA shearing for Next-Gen sequencing, cell lysis, tissue disruption, and compound dissolution.
Focused-ultrasonicators with AFA Technology operate at frequencies well beyond the range of human hearing (500KHz) so, unlike traditional laboratory sonicators, they are inaudible and do not require sound-proof boxes or other special precautions.
The LE220 is also available in the robotic capable (LE220R) configuration.
LE220 utilizes proven industry-standard Covaris Adaptive Focused Acoustic™ AFA™ technology to concurrently shear multiple DNA samples. Non-contact AFA focuses high-frequency acoustic energy into a small, well defined zone within the sample vessel to provide rapid and complete sample processing. Focused acoustic energy provides controlled sample preparation capabilities across a wide range of applications and the isothermal properties of AFA eliminate heat induced molecular changes and sample damage commonly experiences with low frequency bath and probe sonicators.
The Advanced Acoustics of the LE220 utilizes a line-shaped focal zone to expand the effective acoustic field across an entire row or rows of samples in a microplate. This enables the LE220 to process a row of samples in parallel, significantly reducing total AFA processing times.
Covaris AFA technology eliminates operator-induced variation, improves recoveries, increases efficiency, and provides reproducible standardized results.
Designed specifically for High Throughput DNA Fragmentation for sample
Up to 8X higher throughput for targeted applications in a 96 sample format. Faster with 384 sample density and beyond.
Same proven AFA Technology
Highest quality performance, reproducibility, and reliability. Higher recoveries, isothermal, non-contact, controlled energy delivery, random fragmentation.
Compatible platform design
Protocols are easily developed for the LE220.
Process entire rows of samples simultaneously: ensures rapid and consistent sample prep regardless of sample array format.
AFA is inaudible so no sound-proofing or special precautions are needed
Applications / Performance
The LE220 is a versatile instrument that brings industry-standard AFA technology to a wide variety of application areas.
Key applications include:
DNA shearing for Next-Gen Sequencing
Tissue disruption, protein extraction and cell lysis for epigenetics
Dissolution, thawing and mixing for pharma-based compound management applications
As demonstrated in a DNA shearing application below, the LE220 with AFA provides unmatched control and reproducibility.
The unique Covaris line shaped acoustic field enables the LE 220 to provide the same industry proven DNA fragmentation performance as the S2 and E210 workstations.
If you are interested in applying Covaris technologies to your specific application please contact us.