E220 Focused-ultrasonicators

with AFA Technology

The E220 Focused-ultrasonicators are versatile instruments engineered for pre-analytical sample processing by Adaptive Focused Acoustics™ . Utilizing a dish-shaped acoustic transducer and computer controlled electronics; acoustic energy can be tuned to be delivered precisely and accurately to samples of varying volumes.

The E220 enables multi-sample, batch preparation, capable of processing a wide range of sample types and volumes. The E220 may be programed to process from 1 to 96 samples in a single batch. For example, with 96 samples, 96 different AFA energies may be pre-programmed. With the available racks, the E220 instrument is easily configured for use with all AFA Tubes (volumes from 50µl to 10ml) including single AFA microTUBE™ and the AFA 96 microTUBE Plate, for both single and batch sample processing. The E220 can be operated as a stand-alone unit, or easily integrated as part of an automated laboratory system.

The versatility of the E-Series Focused-ultrasonicator makes it possible to bring the advantages of AFA  to numerous biological and chemical applications including, DNA and chromatin shearing, tissue homogenization, cell lysis, compound dissolution, and particle micronization.

Focused-ultrasonicators operate at frequencies well beyond the range of human hearing (500KHz) so, unlike traditional (low frequency) laboratory sonicators they are inaudible and do not require sound-proof boxes or other special precautions.

The E220 is also available in higher power (E220X) and robotic capable (E220R) configurations.


E220 Focused-ultrasonicator

E-Series Datasheet

Covaris Adaptive Focused Acoustics provides unmatched energy control across a wide range of sample prep applications.

Key Features Benefits
Isothermal processing No heat damage, higher recovery
Non-contact, closed vessel No cross contamination, no aerosols, no clean up
Precisely controlled and adjustable energy delivery Standardized processes, highly reproducible
Automation ready Integrates into customers’ sample processing work flow
Multi-sample capability Higher productivity, less operator intervention
Broad sample volume range One instrument for multiple applications

Broad Range of Applications
In life science and pharmaceutical research, the following are some of the areas E-Series are used:

DNA fragmentation Compound formulation
Chromatin shearing High-throughput chemistry
RNA Extraction Tissue disruption and homogenization
Cell spore and organelle Lysis Sample extraction for metabolic and proteomic profiling
Nanoparticle formation – micronization ADME/Tox extractions
Compound dissolution


If you are interested in applying Covaris technologies to your specific application please contact us.

Below is a brief listing of highlighted references:

For a comprehensive list of references please see our reference section.

Technical Specification Description
Model E220 Focused-ultrasonicator
Treatment System Bench-top; high intensity acoustic transducer, temperature monitoring device, circulation pump, water bath with safety enclosure
Treatment Power 100 Watts Average Power
Dimensions 24” (width) x 30” (depth) x 19” (height) (61cm x 76cm x 48cm)
Weight Approximately 110 lbs (50 Kg)
Power Requirements 100-240 VAC 500 VA, 50-60 Hz
Operating Conditions:
Ambient Temperature 15 to 32C
Water Bath Temperature +5C to +40C; Controllable
Water Distilled or deionized water only
Computer: Notebook compatible; available with system from Covaris
Operating System Microsoft Windows XP SP3 minimum or Windows 7
Regulatory Certifications CE, ETL Mark (for Product Safety), WEEE
Safety Complies with Low Voltage Directive 2006/95/EC. Certified to IEC/EN/UL 61010-1:2004 “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part1: General Requirements”
EMC Complies with radio frequency emissions and immunity requirements for Class A Industrial/Scientific/Medical (ISM) equipment under EN 61326-1:2005, as well as EN 61000-3-2:2004 and EN61000-3-3:1995. These standards meet the essential requirements of the EU EMC Directive 2004/108/EC. Additionally, the instrument meets FCC Part 15 Class A radio emissions requirements for the USA and ICES-003 Class A for Industry Canada.