Optimizing ChIP for Reproducibility

Chromatin Immunoprecipitation (ChIP) is a powerful technique for evaluating the interactions
of proteins with specific regions of genomic DNA, helping to better understand the mechanisms
such as, gene regulation, DNA replication, repair and recombination, and epigenetic silencing.
The use of ChIP in conjunction with NGS (ChIP-Seq) has enabled wide scale application of this
technique to ascertain genome wide DNA binding sites. However, sample preparation for ChIP
and ChIP-seq has multiple steps which are critical to the success of the experiments and affect
the reproducibility, bias, and sensitivity of the technique.

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