Entries by radar

Protein Extraction from Yeast: Comparison of the Covaris Adaptive Focused Acoustics™ (AFA) Process to Conventional Bead Beating and Probe Sonication

The efficiency of several mechanical-based lysis and extraction techniques, such as Adaptive Focused Acoustics (AFA), probe sonication, and bead beating from yeast isolates was compared for (i) total protein yield, (ii) preservation of enzymatic activity, (iii) fragmentation of proteins, and (iv) protein bias (i.e., the failure to isolate specific proteins). Protein bias was determined from […]

Effective disruption of Pseudomonas aeruginosa biofilms using Adaptive Focused Acoustics™ (AFA)

Adaptive Focused Acoustics (AFA) was used to effectively disperse Pseudomonas aeruginosa biofilms and disrupt bacterial cells in a low detergent concentration lysis buffer, significantly increasing the efficiency of protein extraction. Biofilm specific staining with Calcoflour White M2R revealed that overnight cultures of Pseudomonas aeruginosa PA14 produce extensive biofilms, and counterstaining with the cell permeable SYTO […]