This document provides users with a complete set of protocols that encompass all Covaris Adaptive Focused Acoustics™ (AFA) instruments for DNA shearing to 150 to 200 bp with 50 to 55 µl of sample volume, to be readily compatible with the Agilent SureSelect Target Enrichment System (XT or XT2). The goal is to provide the most […]
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Entries by Covaris
DNA Shearing with ME220 Focused-ultrasonicator Quick Guide
Safety Data Sheet for B3 Buffer
Safety Data Sheet for the AFA Conditioning Buffer
This work describes a protocol for chromatin immunoprecipitation (ChIP) using a mature mouse T-cell line. This protocol is suitable to investigate the distribution of specific histone marks at specific promoter sites or genome-wide.
OVERVIEW Next-Generation Sequencing (NGS) platforms require controlled generation of DNA fragments to produce high-quality sequencing data. Covaris leverages its proprietary and patented Adaptive Focused Acoustics (AFA™) technology to convert focused high frequency acoustic energy into mechanical force to randomly fragment nucleic acids in an isothermal and non-contact environment.
PN 010255 Adaptive Focused Acoustics™ (AFA)-based chromatin shearing for ChIP-based applications for Products PN 520156 and PN 520158
Covaris provides tools and technologies to improve pre-analytical sample preparation, enable novel drug formulations, and manage compounds in the drug discovery process.